Development of sediment reference sample for toxicity tests

Classification[ edit ] Classically, "lead poisoning" or "lead intoxication" has been defined as exposure to high levels of lead typically associated with severe health effects.

Development of sediment reference sample for toxicity tests

It might be one of the toxic sensitizer and irritant on the skin, and initiator of lung diseases, so it is important to understand the characterization and toxic mechanism of MI in the body.


In the pharmacokinetic study using rats, presented analysis method was useful for detecting the profile of MI in the plasma. We determined half-life, clearance and distribution of MI by WinNonlin software and these might be useful information for the study on the toxicokinetics in body.

In spite of the introduction of new agents such as L-LAAM and buprenorphine, methadone continues to be the drug of choice for the treatment of heroin addiction. Due to the wide variation in half-life among individuals reported values hoursthere is a significant ovedose risk.

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Together with the requirement for effective monitoring of replacement therapy, it emphasizes the need for development of fast, precise and reliable analytical method for quantification of methadone in biological samples.

In this research, liquid chromatography-mass spectrometry LC-MS method for determination of methadone in blood was developed, using solid-phase SPE extraction for the preparation of samples.

The chromatographic separation was performed on XTerra MS C18 column, using a mixture of formic buffer pH 3,5 — acetonitrile Linearity was achieved in the range from 0. Retention time of methadone was 5. Limit of detection and limit of quantification were 1.

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The intra-assay precisions RSD were about 0. The inter-assay precisions RSD were 0. The accuracy varied from Described LC-MS method for analysis of methadone in blood is precise, accurate, reproducible and reliable, with significantly shortened time of analysis run time 10 min.

Development of sediment reference sample for toxicity tests

Due to low therapeutic index of carbamazepine, there is a need for routine measuring its concentrations in serum. Monitoring of the drug level helps to adjust the dose and achieve optimal therapeutic effects while avoiding subtherapeutic and toxic level.

The aim of this study was to determine serum concentration of carbamazepine in patients with epilepsy who are on monotherapy, as well as to show the correlation of different methods on different systems for determination of the drug in serum spectrophotometric and chromatographic method.

For the study were used serums of the patients with epilepsy, 35 serums total. Concentration of carbamazepine in serum, in the interval of reference values had Our results show that the most of patients on carbamazepine therapy have the correct dosage regimen.

The statistical difference between different systems and methods is not significant. There is a strong correlation and connection, so the photometric method is reliable and applicable for routine operation. Box3Institute of general and physical chemistry, P.toxicity and bioaccumulation tests.

As part of USEPA‘s Contaminated Sediment Management Strategy, all Agency programs have agreed to use the same methods to determine whether sediments have the. Caesium (IUPAC spelling) or cesium (American spelling) is a chemical element with symbol Cs and atomic number It is a soft, silvery-gold alkali metal with a melting point of °C ( °F), which makes it one of only five elemental metals that are liquid at or near room temperature.

Caesium has physical and chemical properties similar to those of rubidium and potassium. Reference-toxicity test is a test conducted in conjunction with sediment tests to determine possible changes in condition of the test organisms.

Deviations outside an established normal range indi- cate a change in the . Dec 15,  · Specific gravity is a measurement of urine concentration and is representative of the kidney’s ability to concentrate urine. The specific gravity is a comparison of the amount of solutes in urine as compared with pure water.

[] Specific gravity may also be used as a rough estimate of urine osmolality.

Development of sediment reference sample for toxicity tests

Overlying water for the sediment toxicity tests was collected from approximately m above the stream bed at an upstream reference area and shipped overnight to the toxicity testing laboratory, as needed. This research included (1) analysis of NPDES-compliance data (+ tests), (2) two interlaboratory testing programs, (3) analysis of reference-fluid data from one commercial laboratory, and (4) refinements to test sediment type and effects on animal health.

ASTM E - 07 Standard Guide for Conducting Whole Sediment Toxicity Tests with Amphibians